- Glossinidae (Tsetse flies)
- Diet: Blood-feeding, primarily on mammals.
- Breeding: Viviparous; females give birth to fully developed larvae, which pupate in soil.
- Size: 6–14 mm.
- Marginidae
- Diet: Likely detritivorous or associated with decaying organic matter.
- Breeding: Limited data; likely oviparous with larvae developing in moist habitats.
- Size: Small to medium-sized.
- Mormotomyiidae (Mormotomyia hirsuta, the “terrible hairy fly”)
- Diet: Thought to be associated with bat guano or decaying organic matter.
- Breeding: Found in rock crevices; larvae likely develop in guano-rich environments.
- Size: 1–3 mm.
- Natalimyzidae
- Diet: Unknown, but likely associated with decaying vegetation or fungi.
- Breeding: Limited data; likely oviparous.
- Size: Under 5 mm.
- Tephritidae (True fruit flies)
- Diet: Many species feed on fruit, some are agricultural pests.
- Breeding: Oviparous; larvae develop inside fruit.
- Size: 3–10 mm.
- Tabanidae (Horseflies)
- Diet: Blood-feeding (females), nectar-feeding (males).
- Breeding: Larvae develop in aquatic or moist environments.
- Size: 10–25 mm.
- Calliphoridae (Blowflies)
- Diet: Scavengers, some species parasitic.
- Breeding: Oviparous; larvae develop in decaying matter or wounds.
- Size: 4–12 mm.
- Stratiomyidae (Soldier flies)
- Diet: Larvae feed on decaying organic matter; adults nectar-feed.
- Breeding: Oviparous; larvae develop in soil or aquatic habitats.
- Size: 5–20 mm.
- Bombyliidae (Bee flies)
- Diet: Adults nectar-feed; larvae parasitize other insects.
- Breeding: Larvae develop inside host insects.
- Size: 3–15 mm.
- Asilidae (Robber flies)
- Diet: Predatory, feeding on other insects.
- Breeding: Larvae develop in soil or decaying wood.
- Size: 10–30 mm.
- Dolichopodidae (Long-legged flies)
- Diet: Predatory, feeding on small insects.
- Breeding: Larvae develop in moist environments.
- Size: 2–10 mm.
- Empididae (Dance flies)
- Diet: Predatory, feeding on small insects.
- Breeding: Larvae develop in leaf litter or aquatic habitats.
- Size: 3–12 mm.
- Syrphidae (Hoverflies)
- Diet: Adults nectar-feed; larvae vary from predatory to detritivorous.
- Breeding: Larvae develop in decaying matter or aphid colonies.
- Size: 5–15 mm.
- Phoridae (Scuttle flies)
- Diet: Scavengers, some species parasitic.
- Breeding: Larvae develop in decaying matter or inside host organisms.
- Size: 1–6 mm.
- Ceratopogonidae (Biting midges)
- Diet: Blood-feeding (females), nectar-feeding (males).
- Breeding: Larvae develop in aquatic or moist environments.
- Size: 1–3 mm.
- Psychodidae (Moth flies)
- Diet: Adults nectar-feed; larvae feed on organic debris.
- Breeding: Larvae develop in moist environments.
- Size: 2–5 mm.
- Chloropidae (Grass flies)
- Diet: Some species feed on plants, others are predatory.
- Breeding: Larvae develop in plant stems or decaying matter.
- Size: 2–8 mm.
- Tachinidae (Parasitic flies)
- Diet: Larvae parasitize other insects; adults nectar-feed.
- Breeding: Larvae develop inside host insects.
- Size: 5–20 mm.
- Muscidae (House flies and relatives)
- Diet: Scavengers, some species parasitic.
- Breeding: Larvae develop in decaying matter or animal waste.
- Size: 4–12 mm.
- Anthomyiidae (Root maggot flies)
- Diet: Larvae feed on plant roots or decaying matter.
- Breeding: Larvae develop in soil or organic debris.
- Size: 3–10 mm.
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